Select Outlines from the 'Show:' popup menu and ImageJ will open a window containing numbered Note that the Circularity field was added in ImageJ 1.35e. A value of 1.0 indicates a perfect circle. The formula for circularity is 4pi(area/perimeter^2). Particles with circularity values outside the range specified in theĬircularity field are also ignored. Enter a single value in Size and particles smaller than that valueĪre ignored. Use the dialog box to configure the particle analyzer. Use Image>Adjust>Threshold to threshold an image. Resumes scanning until it reaches the end of the image or selection. The wand tool, measures it using the Measure command, fills it to make it invisible, then The image or selection until it finds the edge of an object. This command counts and measures objects in binary or thresholded images. The width of the columns in the 'Results' window can be adjusted by clicking on and dragging the vertical Measurements by right-clicking in the Results window and selecting

The clipboard by selecting Edit>Copy All from the 'Results' window menu bar. To export the measurements as a tab-delimited text file, select File>Save As>Measurementsįrom the ImageJ menu bar or File>Save As from the 'Results' window menu bar. The weighting factors can be changed using Used to convert to from RGB to YUV, the color encoding The default weighting factors are the ones RGB pixels are converted toįormula V=(R+G+B)/3, or V=0.299R+0.587G+0.114B if 'Weighted RGB Conversions' isĬhecked in Edit>Option>Conversions. With RGB images, results are calculated using brightness values. RecordsĬoordinates if one or more points have been defined using the point selection tool. Calculates line lengthĪnd angle if a line selection has been created using one of the three line selection tools. Has been selected using one of the first four tools in the tool bar. The image and click to the left of one of the drawn outlines with theīased on the selection type, calculates and displays either area statistics, line lengths and angles,Īrea statistics are calculated if there is no selection or if a subregion of the image Multiple selections can be saved by transferring them to a blank window, drawing each one using Edit/Draw,Īnd saving the resulting image. Selections can be saved to disk using File/Save As/ROI and restored using File/Open. Theshold Gray Levels that correspond towhat you want to measure: Image / Adjust / Theshold

Pick the Gray scale image that best delimits the cells of interest Use Image / Color / Split Channels to make 3 gray Scale Images from your RGB image You edit the data in the spreadsheet to keep track of tissues, etc. Once you have collected all your measurements, click File / Save As/Īnd save the data as an excel sheet. Repeat for as many regions you want to measure. Select Straight Line, Drag and drop a line to correspond with region toīe measured. are calculated from the values of the pixels along the line. Standard deviation, mode, min, max and bounding rectangle (v1.34l or later). With line selections, the following parameters can be recorded: length, angle (straight lines only), mean, Use the Analyze>Set Measurements command to specify what area statistics are recorded. ImageJ will have automatically filled in the Distance in Pixelsįield based on the length of the line selection. The known distance and unit of measurement, then click OK. Then, bring up the Set Scale dialog, enter Line selection tool to make a line selection that corresponds to Before using this command, use the straight Measurement results can be presented in calibrated units, Use this dialog to define the spatial scale of the active image so Brief Instructions for ImageJ Brief instructions for basic image analysis using ImageJ

Imagej particle analysis